RNA Preparation and Purification
Zymo Research Corporation Direct-zol-96 RNA (2x96 Preps)
by using this product, total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.)The extraction method inactivates viruses and other infectious agents.The procedure is easy: simply apply a sample in TRI Reagent to the Zymo-Spin™ I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is high-quality RNA suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-qPCR, hybridization, etc.
New England Biolabs, Inc. NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module – 100 reactions
This module is part of the non-directional Ultra II RNA workflow, for Illumina- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
- Designed for use in the NEBNext Ultra II non-directional RNA workflow
Zymo Research Corporation Quick-RNA Plant MiniPrep™ Kit (50 Preps)
The kit includes unique technology such as the ZR BashingBead™ Lysis Tubes and features a specially formulated RNA Lysis Buffer. The Zymo-Spin™ IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IICR Column efficiently adsorbs total RNA.The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin™ III-HRC Filters. RNA is eluted in as little as 6 l and is suitable for subsequent procedures including RT-qPCR.
Macherey-Nagel NucleoSpin RNA Columns, 250 columns
NucleoSpin RNA Columns (250) binding columns for the isolation of RNA pack of 250
Research Products International Corp Quick-RNA MiniPrep Plus Kit w/Zymo-Spin IIICG Columns , Capped, & Spin-Away Filters, 200 Preps
The Quick-RNA Miniprep Plus Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
STEMCELL Technologies Total RNA Purification Kit, Size: 50 Preparations
The Total RNA Purification Kit uses a membrane-based system to quickly purify intact total RNA from 1 x 10^2 to 5 x 10^6 cells without an organic extraction step, eliminating the need for additional purification steps. The Total RNA Purification Kit includes an on-column DNase I treatment to significantly reduce genomic DNA contamination and ensure the purified RNA is suitable for sensitive downstream applications. Intact total RNA can be isolated in as little as 30 minutes. The Total RNA Purification Kit is not recommended for the purification of small RNAs (< 200 nt), such as miRNA.
TRIBIOSCIENCE INC Ribosome RNA Purification Kit, 20 Tests
Tribioscience’s Ribosome RNA Isolation Kit provides a streamlined approach to isolating ribosome RNA from different tissues and cells. Kit size is 20 sample preparation
Norgen Biotek Corp Total RNA Purification 96-Well Kit
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses. The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
